Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3)
نویسندگان
چکیده
منابع مشابه
Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3)*
Immunoglobulin G (IgG) is one of the most abundant proteins present in human serum and a fundamental component of the immune system. IgG3 represents ∼8% of the total amount of IgG in human serum and stands out from the other IgG subclasses because of its elongated hinge region and enhanced effector functions. This study reports partial O-glycosylation of the IgG3 hinge region, observed with nan...
متن کاملPrimary Structure of the “Hinge” Region of Human IgG3
The middle part of the heavy chain of IgG3 (hinge region) which covalently links the two y3 chains to each other, is about 4 times larger than the same region in the three other human IgG subclasses. This is probably due to a quadruplication of a 45-nucleotide DNA segment resulting in a y3 hinge region which is 62 amino acid residues long and consists of an NH,-terminal 17-residue segment follo...
متن کاملHeterogeneity of O-glycosylation in the hinge region of human IgA1.
Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry was applied to studies of the molecular heterogeneity of desialylated human IgA1 hinge region glycopeptides released with two IgA1 proteases. Typically, the hinge region of an alpha1 chain contains three to five O-linked glycan chains. Variants of the hinge region peptides released from IgA1(Kni) myeloma pr...
متن کاملUnusual molecular properties of human IgG3 proteins due to an extended hinge region.
The molecular weight of IgG3 myeloma proteins is about 170,000 as judged by gel filtration analyses of the light and heavy chains in 5 M guanidine-1 M acetic acid. The heavy chain of IgG3 has a molecular weight of about 60,000, which is larger by 9,000 to 10,000 than the molecular weight of the heavy chain of IgGl. Under nondenaturing conditions, an apparent molecular weight of 200,000 is obtai...
متن کاملPrimary structure of the "hinge" region of human IgG3. Probable quadruplication of a 15-amino acid residue basic unit.
The middle part of the heavy chain of IgG3 (hinge region) which covalently links the two gamma3 chains to each other, is about 4 times larger than the same region in the three other human IgG subclasses. This is probably due to a quadruplication of a 45-nucleotide DNA segment resulting in a gamma3 hinge region which is 62 amino acid residues long and consists of an NH2-terminal 17-residue segme...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2015
ISSN: 1535-9476
DOI: 10.1074/mcp.m114.047381